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Western Blotting Detection
Amersham ECL Select (Low picogram, <5pg)
Amersham ECL range Áß °¡Àå °¨µµ°¡ ³ôÀº ½Ã¾àÀÔ´Ï´Ù. ´Ü¹éÁú ¹ßÇöÀÇ ÀÛÀº º¯Èµµ °ËÃâ °¡´ÉÇϸç, Low picograms(<5pg) ¼öÁØÀÇ ´Ü¹éÁú±îÁö °ËÃâÇÒ ¼ö ÀÖ½À´Ï´Ù.
Amersham ECL Prime (Mid picogram, 10-100pg)
°í°¨µµ ½Ã¾àÀ¸·Î¼, ½Ã°£¿¡ µû¸¥ ´Ü¹éÁú ¼öÁØÀÇ º¯È¸¦ Á¤È®È÷ °ËÃâÇÒ ¼ö ÀÖµµ·Ï 24½Ã°£ ÀÌ»óÀÇ signal durationÀ» °¡Áý´Ï´Ù.
Amersham ECL (Mid picogram, 20-100pg)
Standard ECL ½Ã¾àÀ¸·Î¼, Mid picograms(20-100pg)¼öÁØÀÇ ´Ü¹éÁú °ËÃâÀÌ °¡´ÉÇÕ´Ï´Ù.
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Amersham¢â ECL¢â Wester Blotting Detection Reagents
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Amersham¢â ECL Select¢â |
100 ml |
RPN2235 |
Amersham¢â ECL¢â Prime |
100 ml |
RPN2232 |
Amersham¢â ECL¢â |
500 ml |
RPN2106 |
250 ml |
RPN2209 |
125 ml |
RPN2109 |
Amersham¢â ECL¢â start |
400 ml |
RPN3244 |
200 ml |
RPN3243 |
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Membrane |
Amersham¢â Hybond¢â P PVDF 0.2 ¥ìm |
260 mm x 4 m |
10600021 |
Amersham¢â Hybond¢â P PVDF 0.45 ¥ìm |
300 mm x 4 m |
10600023 |
Amersham¢â Protran¢â NC 0.2 ¥ìm |
300 mm x 4 m |
10600001 |
Amersham¢â Protran¢â NC 0.4 ¥ìm |
300 mm x 4 m |
10600002 |
Marker |
Amersham¢â ECL DualVue¢â WB Markers |
25 gel loadings |
RPN810 |
Amersham¢â ECL¢â Full-Range Rainbow Markers |
250 ¥ìl |
RPN800E |
HRP conjugated secondary antibodies |
Amersham¢â ECL¢â Mouse IgG HRP-Linked Whole Antibody |
1 ml |
NA931-1ML |
Amersham¢â ECL¢â Rabbit IgG HRP-Linked Whole Antibody |
1 ml |
NA934-1ML |
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Benchmarking data
Amersham ECL Select
Figure 8 shows a side-by-side comparison of Amersham ECL Select with other high-sensitive chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 ¥ìg Primary antibody dilution: 1:10 000 Secondary antibody dilution: 1:100 000 Detection: Amersham Imager 600, 3 min exposure |
Fig 8. Amersham ECL Select shows brighter bands, lower background, and higher limit of detection compared with competitor reagents in Western blotting detection of ERK 1/2.
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Amersham ECL Prime
Figure 6 shows a side-by-side comparison of Amersham ECL Prime with other high-sensitive chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 10 ¥ìg Primary antibody dilution: 1:5000 Secondary antibody dilution: 1:30 000 Detection: Amersham Imager 600, 75 s exposureFig 6. Amersham ECL Prime exhibits similar sensitivity as the competitor reagents in Western blotting detection of ERK 1/2. The Millipore reagents were slightly less sensitivity and also reached saturation at the highest concentrations (10 to 5 ¥ìg), decreasing the dynamic range of these reagents. |
Figure 7 shows Amersham ECL Prime in comparison with Clarity¢â Western ECL Substrate from Bio-Rad.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 ¥ìg Primary antibody dilution: 1:3000 Secondary antibody dilution: 1:30 000 Detection: Amersham Imager 600, 3 min exposureFig 7. Amersham ECL Prime exhibits higher sensitivity and signal intensity compared with Clarity Western ECL Substrate in Western blotting detection of ERK 1/2. |
Amersham ECL
Figure 5 shows a side-by-side comparison of Amersham ECL reagent with other chemiluminescence detection reagents in the same sensitivity range.Samples: Two-fold dilution series of HeLa cell lysate starting at 5 ¥ìg Primary antibody dilution: 1:1000 Secondary antibody dilution: 1:10 000 Detection: Amersham Imager 600, 1 min exposure |
Fig 5. Amersham ECL reagent exhibits similar sensitivity as the competitor reagents in Western blotting detection of ERK 1/2.
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