Western Blotting Detection

Amersham ECL Select (Low picogram, <5pg)
Amersham ECL range 중 가장 감도가 높은 시약입니다. 단백질 발현의 작은 변화도 검출 가능하며, Low picograms(<5pg) 수준의 단백질까지 검출할 수 있습니다.

Amersham ECL Prime (Mid picogram, 10-100pg)
고감도 시약으로서, 시간에 따른 단백질 수준의 변화를 정확히 검출할 수 있도록 24시간 이상의 signal duration을 가집니다.

Amersham ECL (Mid picogram, 20-100pg)
Standard ECL 시약으로서, Mid picograms(20-100pg)수준의 단백질 검출이 가능합니다.

Amersham™ ECL™ Wester Blotting Detection Reagents

품명	규격	품번
Amersham™ ECL Select™	100 ml	RPN2235
Amersham™ ECL™ Prime	100 ml	RPN2232
Amersham™ ECL™	500 ml	RPN2106
	250 ml	RPN2209
	125 ml	RPN2109
Amersham™ ECL™ start	400 ml	RPN3244
	200 ml	RPN3243

관련 제품

구분	품명	규격	품번
Membrane	Amersham™ Hybond™ P PVDF 0.2 μm	260 mm x 4 m	10600021
	Amersham™ Hybond™ P PVDF 0.45 μm	300 mm x 4 m	10600023
	Amersham™ Protran™ NC 0.2 μm	300 mm x 4 m	10600001
	Amersham™ Protran™ NC 0.4 μm	300 mm x 4 m	10600002
Marker	Amersham™ ECL DualVue™ WB Markers	25 gel loadings	RPN810
	Amersham™ ECL™ Full-Range Rainbow Markers	250 μl	RPN800E
HRP conjugated secondary antibodies	Amersham™ ECL™ Mouse IgG HRP-Linked Whole Antibody	1 ml	NA931-1ML
	Amersham™ ECL™ Rabbit IgG HRP-Linked Whole Antibody	1 ml	NA934-1ML

Benchmarking data

Amersham ECL Select

Figure 8 shows a side-by-side comparison of Amersham ECL Select with other high-sensitive chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 μg Primary antibody dilution: 1:10 000 Secondary antibody dilution: 1:100 000 Detection: Amersham Imager 600, 3 min exposure

Fig 8. Amersham ECL Select shows brighter bands, lower background, and higher limit of detection compared with competitor reagents in Western blotting detection of ERK 1/2.

Amersham ECL Prime

Figure 6 shows a side-by-side comparison of Amersham ECL Prime with other high-sensitive chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 10 μg Primary antibody dilution: 1:5000 Secondary antibody dilution: 1:30 000 Detection: Amersham Imager 600, 75 s exposureFig 6. Amersham ECL Prime exhibits similar sensitivity as the competitor reagents in Western blotting detection of ERK 1/2. The Millipore reagents were slightly less sensitivity and also reached saturation at the highest concentrations (10 to 5 μg), decreasing the dynamic range of these reagents.

Figure 7 shows Amersham ECL Prime in comparison with Clarity™ Western ECL Substrate from Bio-Rad.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 μg Primary antibody dilution: 1:3000 Secondary antibody dilution: 1:30 000 Detection: Amersham Imager 600, 3 min exposureFig 7. Amersham ECL Prime exhibits higher sensitivity and signal intensity compared with Clarity Western ECL Substrate in Western blotting detection of ERK 1/2.

Amersham ECL

Figure 5 shows a side-by-side comparison of Amersham ECL reagent with other chemiluminescence detection reagents in the same sensitivity range.Samples: Two-fold dilution series of HeLa cell lysate starting at 5 μg Primary antibody dilution: 1:1000 Secondary antibody dilution: 1:10 000 Detection: Amersham Imager 600, 1 min exposure

Fig 5. Amersham ECL reagent exhibits similar sensitivity as the competitor reagents in Western blotting detection of ERK 1/2.