Ficoll™ PM400

Cell isolation

4–30°C

Only available for 17030005, 17030008

0.12 EU/mg

Spray-dried powder

White

1.2 g/ml

5 years

~ 17 ml/g

~ 5 × 10~⁵

~ 3 × 10⁵

Solid

The reactivity and stability of Ficoll™ PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll™ PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided

"5 ηr at 20°C: 10% (w/v), 20 ηr at 20°C: 20% (w/v), 60 ηr at 20°C : 30% (w/v), 180 ηr at 20°C : 40% (w/v), 600 ηr at 20°C : 50% (w/v)"

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

50°-65°

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

~ 10 nm